Greenup is a home rule-class city located at the confluence of the Little Sandy River with the Ohio River in Greenup County, Kentucky, in the United States. The population was 1,188 at the 2010 census. Greenup is one of three county seats in the Commonwealth of Kentucky to share its name with its county; the other two being Harlan and Henderson.Greenup is a part of the Huntington-Ashland, WV-KY-OH Metropolitan Statistical Area. As of the 2010 census, the MSA had a population of 287,702.